| Autor: |
George S, Themistocleous, Haralampos A, Katopodis, Lubna, Khaldi, Apostolos, Papalois, Charles, Doillon, Antigone, Sourla, Panayotis N, Soucacos, Michael, Koutsilieris |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
In vivo (Athens, Greece). 21(1) |
| ISSN: |
0258-851X |
| Popis: |
Three-dimensional (3-D) type I collagen gel culture systems allow long-term growth of osteoblast-like cells, in vitro. Whether the implantation of 3-D collagen systems can stimulate new bone formation was assessed in male rabbits.A 10-mm segmental diaphyseal defect was surgically produced at the left and right limbs of 50 adult male rabbits. The 3-D systems containing MG-63 osteoblast-like cells were implanted at the right-limb defects of all 50 animals. Twenty-five left-limb defects were implanted with 3-D collagen gels containing no MG-63 cells, while the rest were left empty. The bone repair process was serially assessed by radiography for up to 8 weeks and by histological analysis for up to the week 32 post-surgery.Ninety-four per cent (94%) of the right-limb defects, presented radiographic evidence of complete bone-end bridging within 8 weeks. None of the 50 left-limb defects presented radiographic post-implantation evidence of bone-end bridging. The radiographic evidence of the bone-end bridging was corroborated with histological evidence of new bone formation, while the medullar canals were filled with bone marrow elements.Implants of the 3-D collagen gels containing osteoblast-like cells can be used as stable scaffolds allowing the migration/proliferation of the bone regenerating cells in male rabbits. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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