| Autor: |
Evangelia G, Chronopoulou, Georgios, Premetis, Christina, Varotsou, Nikolaos, Georgakis, Elisavet, Ioannou, Nikolaos E, Labrou |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2178 |
| ISSN: |
1940-6029 |
| Popis: |
Dye-ligand affinity chromatography is a widely used technique in protein purification. The utility of the reactive dyes as affinity ligands results from their unique chemistry, which confers wide specificity toward a large number of proteins. They are commercially available, inexpensive, stable and can easily be immobilized. Significant factors that contribute to the successful operation of a dye-ligand chromatography include matrix type, dye-ligand density, adsorption along with elution conditions and flow rate. The present chapter provides protocols for the synthesis of dye-ligand affinity adsorbents as well as protocols for screening, selection, and optimization of a given dye-ligand purification step. The purification of the glutathione transferases from Phaseolus vulgaris on Cibacron Blue 3GA-Sepharose affinity adsorbent is given as an example. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
