Transcriptional regulation of mouse delta-opioid receptor gene: role of Ets-1 in the transcriptional activation of mouse delta-opioid receptor gene
| Autor: | P, Sun, H H, Loh |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Transcriptional Activation
Transcription Genetic Transfection Binding Competitive Cell Line Proto-Oncogene Protein c-ets-1 Mice Genes Reporter Proto-Oncogene Proteins Receptors Opioid delta Animals Deoxyribonuclease I RNA Messenger Promoter Regions Genetic Cells Cultured Binding Sites Base Sequence Proto-Oncogene Proteins c-ets Brain DNA Blotting Northern Precipitin Tests Chromatin Protein Structure Tertiary Cross-Linking Reagents Gene Expression Regulation Plasmids Protein Binding Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 276(48) |
| ISSN: | 0021-9258 |
| Popis: | Previously, we identified a minimum core promoter of the mouse delta-opioid receptor (DOR) gene. The DOR promoter contains an E-box that binds upstream stimulatory factor and is crucial for the DOR promoter activity in NS20Y cells, a mouse neuronal cell line that constitutively expresses DOR. In the present study, we further analyzed the DOR promoter in NS20Y cells and have demonstrated that transcription factor Ets-1 binds to an Ets-1-binding site overlapping the E-box and trans-activates the DOR promoter by synergizing with upstream stimulatory factor in specific DNA binding. In addition, the Ets-1 DNA-binding domain is sufficient to play the functional role of Ets-1 in trans-activating the DOR promoter. Furthermore, through in vivo cross-linking assays and Northern blot analyses, we have demonstrated that Ets-1 binds to the DOR promoter in the neonatal mouse brain and that overexpressed Ets-1 can significantly enhance the expression of DOR mRNA in primary neonatal mouse neuronal cells. Collectively, our data suggest that Ets-1 functions as a trans-activator of the DOR promoter in the neonatal mouse brain and thus may contribute to the development of the mouse brain DOR system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|