Discordant rifampicin susceptibility results are associated with Xpert
| Autor: | R H, Berhanu, K, Schnippel, R, Kularatne, C, Firnhaber, K R, Jacobson, C R, Horsburgh, C K, Lippincott |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Microbial Sensitivity Tests Mycobacterium tuberculosis biochemical phenomena metabolism and nutrition bacterial infections and mycoses Article South Africa Tuberculosis Multidrug-Resistant polycyclic compounds bacteria Humans Tuberculosis heterocyclic compounds Female Rifampin Antibiotics Antitubercular Nucleic Acid Amplification Techniques Retrospective Studies |
| Zdroj: | The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease. 23(3) |
| ISSN: | 1815-7920 |
| Popis: | SETTING: Xpert® MTB/RIF is the first-line diagnostic test for Mycobacterium tuberculosis and rifampicin (RIF) resistance in South Africa. OBJECTIVE: To describe the rates of Xpert RIF resistance not confirmed on follow-up testing, as well as the patient and test characteristics associated with discordant results. DESIGN: Retrospective review of patients with isolates showing Xpert RIF resistance. Line-probe assay, phenotypic drug susceptibility testing or repeat Xpert were all considered confirmatory tests of RIF resistance. ‘Discordance’ was defined as a patient with RIF resistance identified on initial Xpert testing, with a subsequent confirmatory test indicating RIF susceptibility. Associations were analysed using Pearson χ(2) difference of proportions and modified Poisson regression. RESULTS: RIF discordance occurred in 22/263 subjects and was associated with Xpert probe B, probe binding delay, as opposed to probe dropout, and probe binding delays (ΔCt) of between 4 and 4.9. CONCLUSION: Discordant RIF resistance was common in our cohort and was associated with Xpert probe delay and use of probe B. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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