| Autor: |
N P, Maksimova, R A, Zheldakova, Iu K, Fomichev |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Mikrobiologiia. 51(4) |
| ISSN: |
0026-3656 |
| Popis: |
A technique is proposed for the preparation of Pseudomonas putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing mono-and divalent cations. Pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 M) for 90 min at the pH 7.9 to 8.0 is a prerequisite for the formation of 'protoplasts'. Under these conditions, the yield of 'protoplasts' reached 99.9% of the total cell number. The protoplast-like structures are capable of reversing into the original bacterial forms in a minimal hypertonic medium containing amino acids which are components of the cell wall. The level of reversion reaches 10% for certain strains. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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