Double-negative (CD4- CD8-) T cells with an alpha/beta T cell receptor. Non-MHC-restricted cytolytic activity and lymphokine production
| Autor: | E G, Brooks, D P, Wirt, R M, Goldblum, S, Vaidya, M T, Asuncion, J C, Patterson, C F, Ware, G R, Klimpel |
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| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytotoxicity Immunologic Male Immunity Cellular Lymphokines CD3 Complex Tumor Necrosis Factor-alpha CD8 Antigens Immunologic Deficiency Syndromes Receptors Antigen T-Cell Infant Blotting Northern Flow Cytometry Lymphocyte Activation Major Histocompatibility Complex Interferon-gamma Antigens CD CD4 Antigens Humans T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 144(12) |
| ISSN: | 0022-1767 |
| Popis: | T cell lines with a novel phenotype (CD3+ TCR-alpha/beta+ CD4- CD8-) were developed from the peripheral blood of a patient with a combined immunodeficiency and tissue injury resembling graft-vs-host disease. One of these IL-2-dependent T cell lines demonstrated non-MHC-restricted cytolytic function against tumor targets, syngeneic and allogeneic fibroblasts, and PHA blasts from allogeneic donors. The other cell line only became cytotoxic in the presence of lectin or anti-CD3 antibody. The two cell lines also differed in their expression of the T-200 gene products CD45RO (gp180) and CD45RA (gp220). Both cell lines produced tumor necrosis factor-alpha and -beta and IFN-gamma activity when activated with mitogens or PMA and IL-1. The in vitro functions of these T-cell lines suggest a potential role for alpha/beta double-negative T lymphocytes in tissue injury resembling graft-vs-host disease. |
| Databáze: | OpenAIRE |
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