Association of granulocyte transmigration with structural and cellular parameters of injury in experimental radiation enteropathy
| Autor: | K K, Richter, M K, Fagerhol, J C, Carr, J M, Winkler, C C, Sung, M, Hauer-Jensen |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Dose-Response Relationship Radiation Enzyme-Linked Immunosorbent Assay Blood Proteins Fibrosis Sensitivity and Specificity Rats Rats Sprague-Dawley Disease Models Animal Feces Radiation Injuries Experimental Cell Movement Culture Techniques Intestine Small Confidence Intervals Animals Intestinal Mucosa Biomarkers Granulocytes Interleukin-1 |
| Zdroj: | Radiation oncology investigations. 5(6) |
| ISSN: | 1065-7541 |
| Popis: | Inflammatory cells are involved in the pathogenesis of tissue injury through release of cytokines and biologically active compounds. This study used a novel, noninvasive method to assess the association between granulocyte transmigration and structural and molecular changes in radiation enteropathy. A 4 cm loop of rat small intestine was exposed to 0, 2.8, 12, or 23 Gy localized irradiation. Feces was collected in metabolic cages before and 3, 7, 14, 28, and 42 days after irradiation. Granulocyte marker protein (GMP) was measured in buffer extracts of feces by enzyme-linked immunosorbent assay (ELISA). Irradiated and shielded intestine were procured at 2 and 26 weeks and assessed for histopathologic injury [radiation injury score (RIS)], ED-2 positive macrophages, and interleukin-1 alpha (IL-1 alpha) positive cells. Irradiated intestine exhibited characteristic histopathologic alterations and increased numbers of macrophages and IL-1 alpha positive cells. There was a highly significant dose-dependent increase in post-radiation GMP (P0.0001). Maximal GMP excretion occurred 3-7 days after irradiation. Six weeks after irradiation, GMP excretion had returned to normal in the 2.8 and 12 Gy groups, but was still 3.5 times higher in the 23 Gy group than in controls. The associations between early GMP excretion and RIS and fibrosis at 26 weeks were highly significant (P0.001 and P0.0001, respectively). Post-radiation granulocyte transmigration is dose-dependent and correlates with structural and molecular changes, as well as with subsequent chronic injury. The GMP assay is a sensitive, non-invasive indicator of acute intestinal radiation injury and a promising biological predictor of chronic toxicity. Our data underscore the importance of consequential mechanisms in radiation enteropathy. |
| Databáze: | OpenAIRE |
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