| Autor: |
N V, Beliakova, O K, Legina, N L, Ronzhina, I V, Shevelev, V M, Krutiakov |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Izvestiia Akademii nauk. Seriia biologicheskaia. (5) |
| ISSN: |
1026-3470 |
| Popis: |
The possibility of interaction of recombinant proteins of human repair DNA polymerase beta with proofreading 3' --5'-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3' --5' -exonucleases mTREX1 and hTREX2 and DNA polymerase beta. DNA polymerase activity is shown to increase four-fold in the presence of 3' --5'-exonuclease TREX2. The experiments with the use of immunodot and Western blot assays on the binding of DNA-polymerase beta with 3' --5'-exonucleases TREX1 and TREX2 immobilized on a nitrocellulose membrane provided additional evidence on the direct association of the above proteins in complexes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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