The Significance of SDF-1α-CXCR4 Axis in
| Autor: | Yoon-Kyung, Bae, Gee-Hye, Kim, Jae Cheoun, Lee, Byoung-Moo, Seo, Kyeung-Min, Joo, Gene, Lee, Hyun, Nam |
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| Rok vydání: | 2017 |
| Předmět: |
Benzylamines
Receptors CXCR4 perivascular cells Periodontal Ligament Primary Cell Culture Neovascularization Physiologic Mice SCID Cyclams Article Mice angiogenesis Heterocyclic Compounds Osteogenesis Human Umbilical Vein Endothelial Cells Animals Humans mesenchymal stem cells Adipogenesis Tissue Engineering Cell Differentiation Chemokine CXCL12 Antigens Surface Blood Vessels Molar Third Pericytes SDF-1α-CXCR4 axis periodontal ligament stem cells |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 |
| Popis: | Periodontal ligament stem cells (PDLSCs) are multipotent stem cells derived from periodontium and have mesenchymal stem cell (MSC)-like characteristics. Recently, the perivascular region was recognized as the developmental origin of MSCs, which suggests the in vivo angiogenic potential of PDLSCs. In this study, we investigated whether PDLSCs could be a potential source of perivascular cells, which could contribute to in vivo angiogenesis. PDLSCs exhibited typical MSC-like characteristics such as the expression pattern of surface markers (CD29, CD44, CD73, and CD105) and differentiation potentials (osteogenic and adipogenic differentiation). Moreover, PDLSCs expressed perivascular cell markers such as NG2, αsmooth muscle actin, platelet-derived growth factor receptor β, and CD146. We conducted an in vivo Matrigel plug assay to confirm the in vivo angiogenic potential of PDLSCs. We could not observe significant vessel-like structures with PDLSCs alone or human umbilical vein endothelial cells (HU-VECs) alone at day 7 after injection. However, when PDLSCs and HUVECs were co-injected, there were vessel-like structures containing red blood cells in the lumens, which suggested that anastomosis occurred between newly formed vessels and host circulatory system. To block the SDF-1α and CXCR4 axis between PDLSCs and HUVECs, AMD3100, a CXCR4 antagonist, was added into the Matrigel plug. After day 3 and day 7 after injection, there were no significant vessel-like structures. In conclusion, we demonstrated the peri-vascular characteristics of PDLSCs and their contribution to in vivo angiogenesis, which might imply potential application of PDLSCs into the neovascularization of tissue engineering and vascular diseases. |
| Databáze: | OpenAIRE |
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