| Autor: |
Lin, Zhou, Xiao-Li, Liu, Yu-Wu, Li, Liang, Wu, Guang-Zhou, Wang, Zheng-Fang, Wang, Li, Ma, Jun, Guan, Chong-Xu, Han |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 30(5) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the expression level and prognostic value of miR-21, miR-18a, miR-146a, and Let-7b derived from serum exosomes in patients with multiple myeloma (MM).Serum exosomes were extracted from 57 MM patients and 20 healthy persons using ExoQuick exosome precipitation solution kit, and the relative expression level of miR-21, miR-18a, miR-146a, and Let-7b derived from serum exosomes was measured by RT-qPCR. Correlations of the expression levels of all miRNAs mentioned above with routine laboratory parameters were analyzed by Spearman correlation analysis. The relationship between the expression level of miR-21, miR-18a, miR-146a, and Let-7b derived from serum exosomes and overall survival of patients with MM was analyzed using the Kaplan-Meier survival curve.The expression levels of miR-21, miR-18a, and Let-7b derived from serum exosomes in patients with MM were significantly lower than those in the normal control group (P0.001), while the expression level of miR-146a between the two groups was not significantly different (P0.05). The expression level of miR-21 was strongly negatively correlated with serum βAberrant low expression levels of miR-21, miR-18a, and Let-7b derived from serum exosomes exist in patients with MM, which are associated with a worse overall survival rate.血清外泌体来源的miRNAs在多发性骨髓瘤中的临床研究.研究血清外泌体来源的miR-21、miR-18a、miR-146a、Let-7b在多发性骨髓瘤（MM）患者中的表达水平及其在预后评估中的价值。.采用ExoQuick试剂盒提取57例MM患者和20例健康体检者血清外泌体，用RT-qPCR检测血清外泌体中miR-21、miR-18a、miR-146a、Let-7b的相对表达水平，采用Spearman相关性分析研究miR-21、miR-18a、miR-146a、Let-7b表达水平与常规实验室指标之间的相关性，Kaplan-Meier生存曲线分析miR-21、miR-18a、miR-146a、Let-7b的表达水平与MM患者总体生存的关系。.MM患者血清外泌体来源的miR-21、miR-18a、Let-7b的表达水平均显著低于正常对照组（P0.001），而miR-146a在两组之间的表达水平无显著性差异（P0.05）。miR-21的表达水平与血清β血清外泌体来源的miR-21、miR-18a、Let-7b在MM患者中存在异常低表达，且与MM患者的总体生存率下降存在关联。. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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