Absence of lymphatic vessels in non-functioning bleb capsules of glaucoma drainage devices
| Autor: | Robert, Siggel, Falk, Schroedl, Thomas, Dietlein, Konrad R, Koch, Christian, Platzl, Alexandra, Kaser-Eichberger, Claus, Cursiefen, Ludwig M, Heindl |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Adolescent Vesicular Transport Proteins Antigens Differentiation Myelomonocytic Ophthalmologic Surgical Procedures Young Adult Antigens CD Humans Child Glaucoma Drainage Implants Aged Lymphatic Vessels Retrospective Studies Membrane Glycoproteins Macrophages Glaucoma Middle Aged Fibrosis Prosthesis Failure Platelet Endothelial Cell Adhesion Molecule-1 Treatment Outcome Child Preschool Blood Vessels Female Biomarkers |
| Zdroj: | Histology and histopathology. 35(12) |
| ISSN: | 1699-5848 |
| Popis: | To evaluate the presence and appearance of blood and lymphatic vessels in non-functioning bleb capsules of glaucoma drainage devices (GDD).Non-functioning (n=14) GDD-bleb capsules of 12 patients were analyzed by immunohistochemistry for blood vessels (CD31, vascular endothelium), lymphatic vessels (lymphatic vessel endothelial hyaluronan receptor-1 [LYVE-1] and podoplanin) and macrophages (CD68).CD31+++ blood vessels and CD68+ macrophages were detected in the outer layer of all specimens. LYVE-1 immunoreactivity was registered in single non-endothelial cells in 8 out of 14 (57%) bleb capsule specimens. Podoplanin-immunoreactivity was detected in all cases, located in cells and profiles of the collagen tissue network of the outer and/or the inner capsule layer. However, a colocalization of LYVE-1 and podoplanin as evidence for lymphatic vessels was not detected.We demonstrate the presence of blood-vessels but absence of lymphatic vessels in non-functioning bleb capsules after GDD-implantation. While the absence of lymphatic vessels might indicate a possible reason for drainage device failure, this needs to be confirmed in upcoming studies, including animal experiments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|