Simplified northern blot hybridization using 5% sodium dodecyl sulfate

Autor:	G D, Virca, W, Northemann, B R, Shiels, G, Widera, S, Broome
Rok vydání:	1990
Předmět:	Mice Animals Nucleic Acid Hybridization RNA Sodium Dodecyl Sulfate Blotting Northern DNA Probes
Zdroj:	BioTechniques. 8(4)
ISSN:	0736-6205
Popis:	We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c6d013131239cd4a7b96d3cee7d6704a https://pubmed.ncbi.nlm.nih.gov/1692715 Zobrazit plný text záznamu