Tracking Yeast Metabolism and the Crabtree Effect in Real Time via CO

Autor: M Rizwan, Ahmed, Nicholas, Doyle, Cathal, Connolly, Seán, McSweeney, Jacob, Krüse, John, Morrissey, Michael B, Prentice, Dara, Fitzpatrick
Rok vydání: 2019
Předmět:
Zdroj: Journal of biotechnology. 308
ISSN: 1873-4863
Popis: In this study, a new approach to measure metabolic activity of yeast via the Crabtree effect is described. BARDS is an analytical technique developed to aid powder and tablet characterisation by monitoring changes in the compressibility of a solvent during solute dissolution. It is a rapid and simple method which utilises a magnetic stir bar to mix added solute and induce the acoustic resonance of a vessel containing a fixed volume of solvent. In this study it is shown that initiation of fermentation in a yeast suspension, in aqueous buffer, is accompanied by reproducible changes in the frequency of induced acoustic resonance. These changes signify increased compressibility of the suspension due to CO
Databáze: OpenAIRE