| Autor: |
Ming, Shao, Qian-Kun, Yang, Wei-Tao, Zhu, Yong-Kui, Kong, Jie, Song, Jing, Wang, Xian-Ping, Lyu |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(5) |
| ISSN: |
1009-2137 |
| Popis: |
To detect the ABO / RhD blood type of infants younger than 6 months in different gestational age and month old with automatic microcolumn glass sphere and tube method, and compare the result of the two methods.The data of 896 samples of infants younger than 6 months from January 2018 to February 2019 was collected. The two methods were used to detect ABO/RhD blood type in all samples and compare the detection rate of ABO/RhD antigen and ABO reverse typing and agglutination intensity of the two methods.Three hundred and eight cases of type A (34.4%), 281 cases of type B (31.4%), 210 cases of type O (23.4%), 97 cases of type AB (10.8%), and 896 positive cases of RhD blood type were detected out by two methods. There were no significant differences of ABO/RhD antigen agglutination intensity between two methods (P0.05). Except for type AB, the detection rate of ABO reverse typing in infants with type B was significantly higher than that with type A and type O (P0.05). The agglutination intensity of type A reverse cell was higher than type B reverse cell (P0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing in the samples of type A and type O group and agglutination intensity of ABO reverse typing in all types as compared with the tube method (P0.05). The detection rate and agglutination intensity of ABO reverse typing in term group were significantly higher than those in preterm group (P0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing and agglutination intensity compared with the tube method between two groups (P0.05). The detection rate and agglutination intensity of ABO reverse typing in group IV (4-6 months old) were significantly higher than those in groups I, II and III (young than 3 months old) (P0.05). The fully automatic microcolumn glass sphere method exhibited higher detection rate of ABO reverse typing in I, II, III groups and agglutination intensity of ABO reverse typing in the 4 groups compared with the tube method (P0.05).ABO / RhD blood group antigen can be accurated detected in majority of infants, but the detection rate of ABO antibody is related to gestational age and month age of infants. The detection rate and agglutination intensity of the fully automatic microcolumn glass sphere method in ABO reverse typing are higher than those of the tube method, especially for premature infants and children within 3 months old.6月龄内婴幼儿ABO/RhD的血型结果及两种检测方法的对比分析.应用全自动微柱玻璃珠法与试管法检测不同胎龄和月龄的6月龄内婴幼儿ABO/RhD血型并对比结果的差异。.收集2018年1月-2019年2月患儿血标本896例，同时应用以上两种方法检测ABO/RhD血型抗原并进行ABO反定型抗体检出率和凝集强度并进行比较分析。.两种方法正定型均检出A型标本308例（34.4%），B型标本281例（31.4%），O型标本210例（23.4%），AB型标本97例（10.8%），RhD血型均为阳性，ABO/RhD血型抗原的凝集强度无明显差异（P0.05）；除AB型外，B型患儿反定型抗体检出率明显高于A型和O型，反定型A细胞凝集强度均明显高于B细胞（P0.05）。全自动微柱玻璃珠法检测A型和O型患儿的反定型抗体检出率及各血型患儿的反定型凝集强度均明显高于试管法（P0.05）。足月儿组的ABO反定型抗体检出率和凝集强度均明显高于早产儿组 （P0.05）。全自动微柱玻璃珠法检测两组患儿反定型抗体检出率以及凝集强度均明显高于试管法（P0.05）。不同月龄4组患儿中，IV组（4月龄至6月龄）患儿ABO反定型检出率和凝集强度均明显高于I、II、III组（3月龄以下，P0.05）。全自动微柱玻璃珠法检测I、II、III组患儿反定型抗体检出率及各组患儿反定型凝集强度均明显高于试管法（P0.05）。.大部分婴幼儿可以准确检测出ABO/RhD血型抗原，而ABO抗体检出率与胎龄和月龄相关。全自动微柱玻璃珠法对ABO血型的反定型抗体检出率及凝集强度均强于试管法，特别对于早产儿以及3月龄以内的患儿更为可靠和敏感。. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
