High levels of ceruloplasmin in the serum of transgenic mice developing hepatocellular carcinoma
| Autor: | D, Pousset, V, Piller, N, Bureaud, F, Piller |
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| Rok vydání: | 2001 |
| Předmět: |
Carcinoma
Hepatocellular Iron RNA Stability Antithrombin III Liver Neoplasms Ceruloplasmin Mice Transgenic Simian virus 40 Chromatography Ion Exchange Molecular Weight Mice Liver Animals Electrophoresis Gel Two-Dimensional RNA Messenger Transgenes Antigens Viral Tumor Holoenzymes Promoter Regions Genetic Copper Glycoproteins |
| Zdroj: | European journal of biochemistry. 268(5) |
| ISSN: | 0014-2956 |
| Popis: | Transgenic mice expressing the Simian virus 40 large T antigen under the control of the liver-specific human antithrombin-III promoter all develop well-differentiated hepatocellular carcinoma. During tumour development serum ceruloplasmin (Cp) increases gradually until it reaches 30 times control levels in all transgenic mice at 6 months of age. The accumulation of Cp in the serum is due to the increased transcription of the Cp gene as well as to the increase in Cp mRNA stability in the livers of the transgenic mice. One-half of the overproduced Cp is charged with copper and Cp-associated serum oxidase activity increases in parallel with the holo-Cp concentration. Through its ferroxidase activity Cp is involved prominently in iron metabolism. Analysis of copper and iron in serum and liver revealed increased copper levels in the serum of tumour-bearing animals and which increased in parallel with Cp concentration; the amounts of copper in the liver were unchanged. In contrast, serum iron remained constant during tumour development whereas the iron concentration in the livers of the transgenic mice decreased. |
| Databáze: | OpenAIRE |
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