The yeast nuclear pore complex: composition, architecture, and transport mechanism
| Autor: | M P, Rout, J D, Aitchison, A, Suprapto, K, Hjertaas, Y, Zhao, B T, Chait |
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| Rok vydání: | 2000 |
| Předmět: |
nucleocytoplasmic transport
Nuclear Envelope Recombinant Fusion Proteins Fluorescent Antibody Technique Membrane Proteins Nuclear Proteins Biological Transport Saccharomyces cerevisiae Models Biological Fungal Proteins Molecular Weight stomatognathic diseases proteomics nucleoporins Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization nuclear pore complex otorhinolaryngologic diseases Original Article Carrier Proteins Microscopy Immunoelectron Chromatography High Pressure Liquid mass spectrometry |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport. |
| Databáze: | OpenAIRE |
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