| Autor: |
M W, Dooper, A, Timmermans, R, Aalbers, E J, Weersink, J G, de Monchy, H F, Kauffman |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Annals of allergy, asthmaimmunology : official publication of the American College of Allergy, Asthma,Immunology. 74(3) |
| ISSN: |
1081-1206 |
| Popis: |
Enhanced activities of peripheral blood cells are a common characteristic of patients with asthma.Here we tested whether this could be due to a dysfunction in one or more signal transduction systems.The production of 1,2-diacylglycerol (1,2-DAG) and arachidonic acid was compared in mononuclear blood cells from patients with asthma (n = 10) and healthy controls (n = 12).Using three different stimuli (concanavalin A, aluminium fluoride or the calcium ionophore A23187) no difference in the production of both 1,2-DAG and arachidonic acid could be found between patients and controls before allergen challenge. Concanavalin A-induced 1,2-DAG production could be inhibited completely in the presence of isoprenaline; concanavalin A-induced arachidonic acid production, partially. The inhibitory effect of adenylate cyclase activation on the production of 1,2-DAG and arachidonic acid was identical in patients and controls. Following allergen challenge, there was a tendency to an increased production of 1,2-DAG and arachidonic acid in controls, whereas in patients there was a tendency to a decreased production.Enhanced cellular activities found in patients with asthma are not caused by an intrinsic dysfunction in production of 1,2-DAG and arachidonic acid. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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