| Autor: |
Hao, Ou, Guo Ling, Li, Hao Qiang, Wang, Guang Yan, Huang, Geng Yuan, Cai, Zi Cong, Li, Zhen Fang, Wu, Xian Wei, Zhang |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Yi chuan = Hereditas. 41(4) |
| ISSN: |
0253-9772 |
| Popis: |
There are two major pathways, homology-directed repair (HDR) and nonhomologous end joining (NHEJ), involved in double-strand break (DSB) repair. Single-stranded oligodeoxyribonucleotide (ssODN)-mediated homologous recombination repair is commonly used for animal site-directed genome editing, with great scientific and practical value. To improve ssODN-mediated HDR efficiency in the pig genome, we investigated the effect and molecular mechanism of mitogen-activated extracellular signal-regulated kinase (MEK) inhibitor PD0325901 on the HDR efficiency in porcine fetal fibroblasts (PFFs). The results showed that PD0325901 obviously increased the percentage of G |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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