| Autor: |
Yi-ming, Zhang, Ruo-ning, Wang, Yan-li, Li, Dong-min, Gu, Zhi-xiang, Cheng, Jun, Xiong, Wei, De, Bing-ying, Chen |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Ai zheng = Aizheng = Chinese journal of cancer. 21(9) |
| Popis: |
The aim of this study was to express and purify human endostatin and to prepare polyclonal antibody of mouse anti-human endostatin.The cDNA of endostatin was amplified by PCR, then recombined into prokaryotic expression vector and transformed into Escherichia coli BL21 for expression; the mice were immunized with purified products.Prokaryotic expression vector pQE-30 of human endostatin was successfully constructed; the expression product was gained after pQE-30 was transferred into BL21. After purified by Ni affinity chromatography, the product was identified to be a single component by SDS-PAGE. Western blot analysis showed that high titer mouse anti-human endostatin polyclonal antibody was successfully prepared.Highly purified expression product and prepared polyclonal antibody provide the necessary material for further study. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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