| Autor: |
M, Blasko, P, Kuliffay, J, Bízik, V, Zajac, M, Grófová, J, Matoska, V, Thurzo |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Neoplasma. 28(6) |
| ISSN: |
0028-2685 |
| Popis: |
A new cell line (B-25F) obtained from a benign polypoid fibrous lesion of the mucosa of oral cavity is described. The cells at first grew in suspension but after a month of cultivation they began to adhere and subsequently formed a monolayer typical for fibroblastoid cells. Population doubling time both in lower and higher passages was 60 h. Electron microscopy studies failed to detect any viral particles or mycoplasmas. A comparison of cell surface glycoproteins of fibroblasts (B-41FB), fibroma cells (B-25F), and fibrosarcoma cells (B-6FS) was made. These lines share common traits in their surface membranes although distinct differences among the individual lines could be detected. Karyological analysis showed that 64% of cells contained 46 chromosomes. This number veiled both diploid and pseudodiploid karyotypes. An aberrant chromosome, t(13;18?) was found. For isoenzyme comparison of B-41FB, B-25F, B-6FS, and HeLa cell lines the mobility of lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PD) was employed. All these lines had LDH patterns of human cells, and a G-6-PD pattern of phenotype B except that of HeLa cells that had phenotype A. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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