[Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2'- deoxyuridine-containing oligodeoxynucleotides]

Autor: A D, Malakhov, E V, Malakhova, S V, Kyznitsova, I V, Grechishnikova, I A, Prokhorenko, M V, Skorobogatyĭ, V A, Korshun, Iu A, Berlin
Rok vydání: 2000
Předmět:
Zdroj: Bioorganicheskaia khimiia. 26(1)
ISSN: 0132-3423
Popis: Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2'-deoxyuridine 3'-phosphoramidite and a solid support carrying this nucleoside. Oligonucleotides containing one or several modified units have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the complementary sequence.
Databáze: OpenAIRE