Platelet-associated and secreted PAF-acetylhydrolase activity in patients with stable angina: sequential changes of the enzyme activity after angioplasty

Autor:	J, Goudevenos, A D, Tselepis, M P, Vini, L, Michalis, D C, Tsoukatos, M, Elisaf, E, Ninio, D A, Sideris
Rok vydání:	2001
Předmět:	Adult Blood Platelets Male Platelet Aggregation Thrombin In Vitro Techniques Middle Aged Hemostatics Phospholipases A Angina Pectoris 1-Alkyl-2-acetylglycerophosphocholine Esterase Humans Female Angioplasty Balloon Coronary Platelet Activating Factor Aged
Zdroj:	European journal of clinical investigation. 31(1)
ISSN:	0014-2972
Popis:	Platelet-activating factor (PAF), the lipid mediator of inflammation and potent platelet agonist, can be hydrolysed and inactivated by PAF-acetylhydrolase (PAF-AH). We investigated the PAF-AH activity in relation to PAF formation in platelets from patients with stable angina undergoing elective percutaneous transluminal coronary angioplasty (PTCA).Twenty-seven patients with stable angina, undergoing PTCA, and 30 age- and sex-matched controls were studied. The platelet-associated and secreted PAF-AH activity was measured, before PTCA, as well as at 4 h, 48 h and 6 months afterwards. PAF formation by thrombin-stimulated platelets and the platelet aggregation responses to PAF and ADP were also determined.The PAF-AH activity secreted by thrombin-stimulated platelets before PTCA (in pmol/10(9) cells/h) was significantly higher compared to controls (892 +/- 222 vs. 624 +/- 144, P0.001). The enzyme activity was not altered at 4 h after PTCA, but was significantly increased at 48 h (1284 +/- 312, P0.005) to return to the levels observed in the control group 6 months afterwards. Detectable levels of PAF in thrombin-stimulated platelets were found only at 6 months after PTCA. Furthermore, the cell-associated enzyme activity in resting platelets before PTCA was significantly lower compared with controls. Unlike in controls, the platelet-associated enzyme activity in the patient group was not increased after stimulation with thrombin and it was associated by a platelet hyperaggregability to PAF. Both the intact cell-associated activity and the platelet hyper-reactivity to PAF were restored at 6 months after PTCA.Alterations in the platelet PAF-AH activity, which affect the PAF formation in thrombin-stimulated platelets and are associated by an increased aggregatory response to PAF, are observed in patients with stable angina and are completely restored after PTCA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c386ad2988aeb6092d667b7ace9854bb https://pubmed.ncbi.nlm.nih.gov/11168431 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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