| Autor: |
R N, Hull, W R, Cherry, G W, Weaver |
| Rok vydání: |
1976 |
| Předmět: |
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| Zdroj: |
In vitro. 12(10) |
| ISSN: |
0073-5655 |
| Popis: |
A stable epithelial-like pig kidney cell strain has been established. This strain has been carried through more than 300 serial passages, has remained free of microbial and viral contaminants, and has retained a near diploid number of chromosomes. Attempts to produce tumors with these cells in immunosuppressed laboratory animals have been uniformly negative. The cells have grown rapidly in monolayer cultures with a split ratio of 1 to 15 at weekly intervals, but have failed to proliferate in suspension cultures. A subline adapted to growth on serum-free medium 199 has been carried through 145 passages on this medium. Several unusual morphologic features have been observed in these cultures including three-dimensional "domelike" structures. These cells have been found susceptible to some viruses and have been especially useful for viruses of domestic animals. LLC-PK1 cells have produced significant levels of plasminogen activator. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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