| Autor: |
N N, Sokolov, A B, Fitsner, N V, Anikeĭcheva, E B, Khoroshutina, O T, Samko |
| Rok vydání: |
1986 |
| Předmět: |
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| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny. 102(12) |
| ISSN: |
0365-9615 |
| Popis: |
5,5'-dithiobis-2-nitrobenzoic acid, N-ethylmaleimide, and parachloromercuribenzoate have been demonstrated to inhibit the activity of restrictases PaeI and PaeII from Ps. aeruginosa bacterial cells. Restrictase PaeII was more sensitive to the action of thiol-specific reagents, as compared to PaeI. The minimal concentration of reagents for SH-groups that completely inhibited the activity of restrictases PaeI and PaeII was determined. The protective effect against the inhibitory action of 5,5'-dithiobis-2-nitrobenzoic acid on the activity of PaeII was observed after preincubation of these enzymes with phage lambda DNA and Mg2+ cations. It is suggested that restrictase PaeI and PaeII molecules contain SH-groups, essential for the enzymatic activity. They are believed responsible for restrictase binding with DNA substrate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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