DNA analysis of a grandfather-father-son relationship from 300-year-old remains of the Date clan in Japan

Autor:	R, Uchihi, T, Yamamoto, H, Nozawa, K, Tamaki, T, Ozawa, T K, Yamada, Y, Katsumata
Rok vydání:	1998
Předmět:	Male HLA-DP Antigens Base Sequence Genotype Genes MHC Class II Molecular Sequence Data Paternity DNA History 18th Century Polymerase Chain Reaction HLA-DQ alpha-Chains History 17th Century Japan HLA-DQ Antigens Humans Lung HLA-DP beta-Chains Hair
Zdroj:	Nihon hoigaku zasshi = The Japanese journal of legal medicine. 52(2)
ISSN:	0047-1887
Popis:	The grandfather-father-son relationship of the first three lords of the Date clan in Japan was ascertained by HLA-DNA sequencing analysis. From their hairs and dried lung tissue found in ca. 300-year-old remains, DNA was extracted with usual phenol-chloroform method followed by purification with cetyltrimethylammonium bromide (CTAB). Two HLA class II genes, HLA-DQA1 and -DPB1, were amplified by seminested, or dual/triple PCR. The PCR products were cloned and analyzed by automated sequencing. Since the three lords shared a haplotype of DQA1*0301-DPB1*0402, we concluded that there is no inconsistency in their lineage. This is the first case of biological evidence for a historical Samurai family relationship in the 17-18th centuries.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c2f8261974f60c6099a7fe4047a001d2 https://pubmed.ncbi.nlm.nih.gov/9711069 Zobrazit plný text záznamu