In vitro selection of allosteric ribozymes

Autor:	Nicolas, Piganeau
Rok vydání:	2009
Předmět:	Allosteric Regulation Doxycycline SELEX Aptamer Technique Point Mutation RNA Catalytic Aptamers Nucleotide Pefloxacin
Zdroj:	Methods in molecular biology (Clifton, N.J.). 535
ISSN:	1064-3745
Popis:	In vitro selection techniques offer powerful and versatile methods to isolate nucleic acid sequences with specific activities from huge libraries. The present protocol describes an in vitro selection strategy for the de novo selection of allosteric self-cleaving ribozymes responding to virtually any drug of choice. We applied this method to select hammerhead ribozymes inhibited specifically by doxycycline or pefloxacin in the sub-micromolar range. The selected ribozymes can be converted into classical aptamers via insertion of a point mutation in the catalytic center of the ribozyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c2f3934677c41e062cfe35d2a6173cc7 https://pubmed.ncbi.nlm.nih.gov/19377981 Zobrazit plný text záznamu