Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes
| Autor: | Aghasadeghi, Mohammad Reza, Delbaz, Seyed Ali, Sadat, Seyed Mehdi, Siadat, Seyed Davar, Ardestani, Mehdi Shafiee, Rahimi, Pooneh, Bolhassani, Azam, Roudsari, Rouhollah Vahabpour, Bahramali, Golnaz, Motevalli, Fateme, Davari, Mehdi, Vakily, Habib, Salmani, Ali Sharifat, Nobari, Maryam Borhan |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Avicenna Journal of Medical Biotechnology |
| ISSN: | 2008-4625 2008-2835 |
| Popis: | Background Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. Methods In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. Results Frequency of IFN-γ secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg+C/IFA groups (p≤0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤ 0.05). Conclusion Analysis of induced responses revealed the immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-γ secreting splenocytes of immunized mice. |
| Databáze: | OpenAIRE |
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