| Autor: |
Ling-ling, Yang, Hui, Shao, Peng-bo, Yuan, Xiao-yue, Guo, Xiao-wei, Zhang, Yang-yu, Zhao |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences. 43(6) |
| ISSN: |
1671-167X |
| Popis: |
To determine hypoxia-inducible factor 1α (HIF-1α) and its target gene, vascular endothelial growth factor (VEGF) and receptor (VEGFR-1) concentrations in the placentas of the donor and recipient in monochorionic twin pregnancies with twin-twin transfusion syndrome (TTTS).Twenty monochorionic twin pregnancy cases were included in the study (10 with and 10 without TTTS). Tissue protein expressions of HIF-1α,VEGF and VEGFR-1 were determined by using immunohistochemistry. Western blot analysis were used to quantify and compare the protein expression. RT-PCR were used to compare their mRNA expressions.HIF-1α was mainly observed in trophoblastic cells and villi capillaries endothelial cells, and VEGF in trophoblastic cells, endothelial cells and villi stromal cells; VEGFR-1 was mainly observed in villi trophoblastic cells and vascular endothelial cells. The placenta protein and mRNA expression of HIF-1α and its target gene in the donor placenta increased significantly (P0.001) compared with that in the control placenta, but the expression of HIF-1α and its target gene in the recipients tended to be similar in the controls (P0.05). There was no difference between the controls.When the monochorionic twin placenta is formed in the early period, HIF-1α, VEGF and VEGFR-1 are over-expressed, which may affect the placenta angiogenesis and induce TTTS . |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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