| Autor: |
N M, Suraeva, A Iu, Baryshnikov, V I, Fisinin, M I, Prokof'ev |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Izvestiia Akademii nauk. Seriia biologicheskaia. (1) |
| ISSN: |
1026-3470 |
| Popis: |
The methods of transfection ofa plasmid with a reporter gene involving DNA injection into chicken embryonic cells were studied. The parameters of the efficient transfection of chicken blastodermal cells with a foreign gene have been determined (20-24 and up to 40% in culture and embryos, respectively). A high efficiency of transfection of primordial germ cells isolated from the gonads has been obtained after DNA injection into the dorsal aorta of 2.5-day-old chicken embryos. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
