Identification of arabidopsis proteins that interact with the cauliflower mosaic virus (CaMV) movement protein
| Autor: | Z, Huang, V M, Andrianov, Y, Han, S H, Howell |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Recombinant Fusion Proteins
Green Fluorescent Proteins Molecular Sequence Data Arabidopsis Saccharomyces cerevisiae Transfection Fluorescence Viral Proteins Caulimovirus Gene Expression Regulation Plant Two-Hybrid System Techniques Animals Tissue Distribution Amino Acid Sequence Phylogeny Plant Proteins Microscopy Confocal Sequence Homology Amino Acid Protoplasts Plants Genetically Modified Plant Viral Movement Proteins Luminescent Proteins RNA Plant Mutation Sequence Alignment Protein Binding |
| Zdroj: | Plant molecular biology. 47(5) |
| ISSN: | 0167-4412 |
| Popis: | Gene I of cauliflower mosaic virus (CaMV) encodes a protein that is required for virus movement. The CaMV movement protein (MP) was used in a yeast 2-hybrid system to screen an Arabidopsis cDNA library for cDNAs encoding MP-interacting (MPI) proteins. Three different clones were found encoding proteins (MPI1, -2 and -7) that interact with the N-terminal third of the CaMV MP. The interaction in the 2-hybrid system between MPI7 and CaMV MP mutants correlated with the infectivity of the mutants. A non-infectious MP mutant, ER2A, with two amino acid changes in the N-terminal third of the MP failed to interact with MPI7, while an infectious second-site mutant, that differed from ER2A by only a single amino acid change, interacted in the 2-hybrid system. MPI7 is encoded by a member of a large, but diverse gene family in Arabidopsis. MPI7 is related in sequence, size and hydropathy profile to mammalian proteins (such as rat PRA1) described as a rab acceptor. The gene encoding MPI7 is expressed widely is Arabidopsis plants. and in transgenic plants the MPI7:GFP fusion protein is localized in the cytoplasm, concentrated in punctate spots. In protoplasts transfected with CFP:MP and MPI7:YFP, CFP:MP colocalized to some of the sites where MPI7:YFP is expressed. At these sites, fluorescence resonance energy transfer (FRET) between fluorophores was observed indicating an interaction in planta between the CaMV MP and MPI7. |
| Databáze: | OpenAIRE |
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