| Autor: |
J, el Baghdadi, R, Lazraq, A, Benani, M, Naciri, S, Ibrahimy, A, Benslimane |
| Jazyk: |
francouzština |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Bulletin de la Societe de pathologie exotique (1990). 90(5) |
| ISSN: |
0037-9085 |
| Popis: |
We have evaluated the frequency of M. tuberculosis strains which lack IS 6110 among 102 sputa isolated from Moroccan patients. A pair of primers was designed to amplify a 201bp DNA fragment of IS 6110. The amplified DNA was detected by ethidium bromide stained agarose gel electrophoresis and confirmed by southern blot hybridization with a 32P-labelled probe (PMTO2). To detect the presence of amplification inhibitors, an internal control DNA was added in each negative PCR result. Among 102 samples, 6 sputa were negative by PCR-IS 6110 but culture positive. The test of detection of M. tuberculosis for 2/6 sputa by PCR Amplicor amplifying 584 pb of rRNA 16s sequence was positive. RFLP analysis of these 2 strains revealed no bands hybridizing IS 6110 but PCR-Mt 308 was positive. These results confirmed that these M. tuberculosis strains are lacking IS 6110. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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