23,25-Dihydroxyvitamin Dlt;subgt;3lt;/subgt; is liberated as a major vitamin Dlt;subgt;3lt;/subgt; metabolite in human urine after treatment with β-glucuronidase: Quantitative comparison with 24,25-dihydroxyvitamin Dlt;subgt;3lt;/subgt; by LC/MS/MS

Autor: Kazunari, Takada, Yukino, Hagiwara, Moeka, Togashi, Atsushi, Kittaka, Fumihiro, Kawagoe, Motonari, Uesugi, Shoichi, Nishimoto-Kusunose, Tatsuya, Higashi
Rok vydání: 2022
Předmět:
Zdroj: The Journal of steroid biochemistry and molecular biology. 223
ISSN: 1879-1220
Popis: The complete understanding of the excretion of surplus 25-hydroxyvitamin Dlt;subgt;3lt;/subgt; [25(OH)Dlt;subgt;3lt;/subgt;] in humans remains to be accomplished. In our previous study, 24,25-dihydroxyvitamin Dlt;subgt;3lt;/subgt; [24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt;] 24-glucuronide was identified as a major urinary vitamin Dlt;subgt;3lt;/subgt; metabolite, while the glucuronide of 23,25-dihydroxyvitamin Dlt;subgt;3lt;/subgt; [23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt;] is another metabolite of interest but has not been sufficiently evaluated. Although the quantitative analysis of 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; liberated in urine by the treatment with β-glucuronidase (GUS) has been conducted, no information was provided about the amount of the glucuronidated 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; in the urine. In this study, we first developed and validated a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS)-based method for the simultaneous quantification of 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; and 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; liberated in urine by GUS. The analysis of the urine samples revealed that the amount of 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; was almost as much as that of 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt;, in contrast to the fact that the plasma concentration of 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; was much lower than that of 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt;. These results strongly suggested that 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; is more susceptible to glucuronidation and more promptly excreted into urine than 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt;. Furthermore, the amount ratios of 23,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; to 24,25(OH)lt;subgt;2lt;/subgt;Dlt;subgt;3lt;/subgt; in the urine samples did not markedly vary during the day (morning/evening) and even by a week-long vitamin Dlt;subgt;3lt;/subgt; supplementation (1000 IU/body/day). We concluded that the C-23 hydroxylation plays a crucial role in the urinary excretion of surplus 25(OH)Dlt;subgt;3lt;/subgt;.
Databáze: OpenAIRE
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