Localization of the fragile X mental retardation 2 (FMR2) protein in mammalian brain

Autor:	W J, Miller, J A, Skinner, G S, Foss, K E, Davies
Rok vydání:	2000
Předmět:	Male Brain Nuclear Proteins Proteins Antibodies Recombinant Proteins Mice Inbred C57BL Mice Fragile X Syndrome Escherichia coli Trans-Activators Animals Humans Cloning Molecular
Zdroj:	The European journal of neuroscience. 12(1)
ISSN:	0953-816X
Popis:	The transcriptional silencing of the FMR2 gene has been implicated in FRAXE mental retardation. FRAXE individuals have been shown to exhibit learning deficits, including speech delay, reading and writing problems. FMR2 encodes a large protein of 1311 amino acids and is a member of a gene family encoding proline-serine-rich proteins that have properties of nuclear transcription factors. To characterize the expression of the fragile X mental retardation 2 (FMR2) protein, polyclonal antibodies were raised against two regions of the human FMR2 protein and used in immunofluorescence experiments on mouse brain cryosections. Our results demonstrate for the first time that the FMR2 protein is localized in neurons of the neocortex, Purkinje cells of the cerebellum and the granule cell layer of the hippocampus. FMR2 staining is shown to colocalize with the nuclear stain 4,6-diamidino-2-phenylindole (DAPI) confirming that FMR2 is a nuclear protein. The localization of FMR2 protein to the mammalian hippocampus and other brain structures involved with cognitive function is consistent with the learning deficits seen in FRAXE individuals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c24e532070c5e48f9f3b12b3c472b987 https://pubmed.ncbi.nlm.nih.gov/10651894 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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