Histological and immunohistochemical analyses of the chronology of healing process after immediate tooth replantation in incisor rat teeth
| Autor: | Sônia Regina, Panzarini, Roberta, Okamoto, Wilson Roberto, Poi, Celso Koogi, Sonoda, Denise, Pedrini, Paula Ervolino, da Silva, Celia Tomiko Matida Hamata, Saito, Heloísa Fonseca, Marão, Paulo, Sedlacek |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Time Factors Periodontal Ligament Acid Phosphatase Gingiva Root Resorption Epithelium Tooth Apex Animals Rats Wistar Tooth Socket Blood Coagulation Dental Pulp Rupture Wound Healing Odontoblasts Receptor Activator of Nuclear Factor-kappa B Tartrate-Resistant Acid Phosphatase RANK Ligand Osteoprotegerin Immunohistochemistry Rats Incisor Isoenzymes Connective Tissue Dental Pulp Calcification Osteopontin Tooth Replantation Collagen Biomarkers |
| Zdroj: | Dental traumatology : official publication of International Association for Dental Traumatology. 29(1) |
| ISSN: | 1600-9657 |
| Popis: | Dental tissues have special characteristics, and its regenerative capacity is noteworthy. However, understanding the circumstances that lead to regeneration is challenging. In this study, the chronology of the healing process after immediate replantation of rat incisor teeth was examined by histological and immunohistochemical analyses within a 60-day period. Thirty-six male Wistar rats had their maxillary right incisors extracted and replanted after 15 min in saline storage. The rats were sacrificed immediately 3, 7, 15, 28, and 60 days after replantation. The histological analysis showed rupture of the periodontal ligament and formation of a blood clot, which started being replaced by a connective tissue after 3 days. At 7 days, the gingival mucosa epithelium was reinserted and areas of root resorption could be seen. At 15 days, the periodontal ligament was repaired. At 3 days, the pulp presented an absence of the odontoblast layer, which started being replaced by a connective tissue. This tissue suffered gradual calcification, filling the root canal at 28 and 60 days. The root ends were closed. The immunohistochemical analysis revealed greater expression of OP, OPG, and RANK proteins in the initial periods (0 and 3 days), while TRAP expression predominated at 28 and 60 days (P0.05). In conclusion, in delayed tooth replantation, there is great new bone formation activity in the earlier periods of the repair process, while a predominance of bone resorption and remodeling is observed in the more advanced periods. |
| Databáze: | OpenAIRE |
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