Visualization of nitric oxide formation in cell cultures and living tissue

Autor:	N P, Wiklund, H H, Iversen, A M, Leone, S, Cellek, L, Brundin, L E, Gustafsson, S, Moncada
Rok vydání:	1999
Předmět:	Lipopolysaccharides Nitric Oxide Synthase Type III Guinea Pigs Myenteric Plexus Nitric Oxide Synthase Type II Nitric Oxide Synthase Type I Arginine Bradykinin Nitric Oxide Interferon-gamma Mice Animals Humans Cells Cultured Neurons Photons Hypogastric Plexus Macrophages Macrophage Activation Electric Stimulation Luminescent Measurements Indicators and Reagents Luminol Endothelium Vascular Rabbits Nitric Oxide Synthase
Zdroj:	Acta physiologica Scandinavica. 167(2)
ISSN:	0001-6772
Popis:	We have visualized nitric oxide (NO) released from cell cultures and living tissue. NO was visualized by a reaction with luminol and hydrogen peroxide to yield photons which were counted using a microscope coupled to a photon counting camera. Murine macrophages were activated with interferon-gamma (IFN-gamma) and endotoxin (LPS). Cultured endothelial cells were stimulated with bradykinin, and neurones in the guinea-pig myenteric plexus and the rabbit hypogastric nerve trunk were electrically stimulated. There was a marked increase in photons emitted from the cultured cells as well as from the living tissues during stimulation. The stimulation-induced photon emission was markedly reduced by inhibition of nitric oxide synthase (NOS); removal of L-arginine from the medium also decreased photon counts. The present method allowed integration times in the order of minutes to improve signal-to-noise ratio. However, the high sensitivity of this method also makes it possible to generate an image in seconds, allowing the production of real time films. Photon emission was enhanced under conditions known to increase NO production, and diminished in the presence of NO inhibitors. Thus, this method has demonstrated specificity for the L-arginine:NO pathway from a wide range of biological sources such as cultured cells and living tissues, and has the potential for real time imaging of NO formation, with high temporal and spatial resolution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::c13a4e19fa9b9e40681ea92a3d360909 https://pubmed.ncbi.nlm.nih.gov/10571552 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.