| Autor: |
Magali Justina Gómez, Usnayo, Luis Eduardo Coelho, Andrade, Renata Triguenho, Alarcon, Juliana Cardoso, Oliveira, Gustavo Milson Fabrício, Silva, Izidro, Bendet, Rufus, Burlingame, Luis Cristóvão, Porto, Geraldo da Rocha Castelar, Pinheiro |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Revista brasileira de reumatologia. 51(5) |
| ISSN: |
1809-4570 |
| Popis: |
The HLA-DRB1 alleles encoding an amino acid sequence (QKRAA/QRRAA/RRRAA) at position 70 74 of the third hypervariable region of the β1 chain of the HLA-DRB1 gene, called shared epitope (SE), are associated with increased susceptibility to and severity of rheumatoid arthritis (RA) in different populations.To determine the frequency of HLA-DRB1 alleles in Brazilian patients with RA and their association with rheumatoid factor (RF) and anti-citrullinated peptide antibodies (ACPA).Four hundred and twelve patients with RA (ACR 1987) and 215 controls were included. HLA-DRB1 typing was performed by use of polymerase chain reaction (PCR) with specific primers and hybridization with sequence-specific oligonucleotide probe (SSOP). ACPA was measured by use of the ELISA technique and RF by nephelometry. The statistical analysis comprised the chi-square and Student t tests and logistic regression.HLA-DRB1*04:01, *04:04, *04:05 alleles were associated with RA (P0.05); despite the wide confidence interval, it is worth noting the association between the DRB1*09:01 allele and RA (P0.05). HLA-DRB1 SE+ alleles were observed in 62.8% of the patients and in 31.1% of controls (OR 3.62; P0.001) and were associated with ACPA (OR 2.03; P0.001). DRB1-DERAA alleles showed a protective effect against RA (OR 0.42; P0.001).In a sample of Brazilian patients with RA, most of whom of mixed heritage, HLA-DRB1 SE+ alleles were associated with susceptibility to disease and presence of ACPA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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