| Autor: |
C, Perrin, V A, Vu, N, Matthijs, M, Maftouh, D L, Massart, Heyden Y, Vander |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Journal of chromatography. A. 947(1) |
| ISSN: |
0021-9673 |
| Popis: |
A strategy for rapid screening for the separation of chiral molecules of pharmaceutical interest by normal-phase liquid chromatography using three cellulose/amylose stationary phases is proposed. In a first step, the most important parameters for the separations were determined and studied for their effects by means of experimental designs. Results showed that the cellulose tris-(3,5-dimethylphenylcarbamate), the amylose tris-(3,5-dimethylphenylcarbamate) and the cellulose tris-(4-methylbenzoate) stationary phases have very broad and complementary enantiorecognition properties. The type of organic modifier used in the mobile phase appeared to have a dramatic influence on the quality of the separation. Based on the results of the preliminary study, a screening strategy was developed and successfully applied to a set of 36 diverse drugs. Enantiomeric separation was observed in 89% of cases and the analysis times were usually shorter than 20 min. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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