Differential processing of influenza nucleoprotein in human and mouse cells
| Autor: | V M, Braud, A J, McMichael, V, Cerundolo |
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| Rok vydání: | 1998 |
| Předmět: |
Antigen Presentation
Viral Core Proteins Mast-Cell Sarcoma RNA-Binding Proteins Biological Transport HLA-A3 Antigen Nucleocapsid Proteins Endoplasmic Reticulum Transfection Epitopes Mice L Cells Nucleoproteins ATP Binding Cassette Transporter Subfamily B Member 3 Influenza A virus Tumor Cells Cultured Animals Humans ATP-Binding Cassette Transporters ATP Binding Cassette Transporter Subfamily B Member 2 Antigens Viral T-Lymphocytes Cytotoxic |
| Zdroj: | European journal of immunology. 28(2) |
| ISSN: | 0014-2980 |
| Popis: | To investigate how early events in antigen processing affect the repertoire of peptides presented by MHC class I molecules, we compared the presentation of the influenza A nucleoprotein epitope 265-273 by HLA-A3 class I molecules in human and mouse cells. Mouse cells that express HLA-A3 failed to present the NP265-273 peptide when contained within the full-length nucleoprotein, to HLA-A3-restricted human cytotoxic T lymphocytes. However, when the epitope was generated directly in the cytosol using a recombinant vaccinia virus that expressed the nonamer peptide, mouse cells were recognized by HLA-A3-restricted CTL. Poor transport of the peptide by mouse TAP was not responsible for the defect as co-infection of mouse cells with recombinant vaccinia viruses encoding the full-length nucleoprotein and the human TAP1 and TAP2 peptide transporter complex failed to restore presentation. These results therefore demonstrate a differential processing of the influenza nucleoprotein in mouse and human cells. This polymorphism influences the repertoire of peptides presented by MHC class I molecules at the cell surface. |
| Databáze: | OpenAIRE |
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