| Autor: |
R V, Chari, J W, Kozarich |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. 256(19) |
| ISSN: |
0021-9258 |
| Popis: |
Fluoromethylglyoxal in the presence of glutathione has been shown to undergo a novel glyoxalase I-catalyzed product partitioning to S-fluorolactoylglutathione and S-pyruvylglutathione with fluoride elimination. While the partition ratio (fluoride eliminated/total fluoride) was insensitive to pH 5.5-7.5 and concentrations of substrate and glyoxalase I, it was species-dependent. When [1-2H]fluoromethylglyoxal was reacted with glyoxalase I, an increase in the partition ratio and retention of deuterium in the product fluorolactate was observed. This result can only be explained by a selective primary isotope effect on the protonation of an enediol intermediate relative to fluoride elimination. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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