| Autor: |
Yanzhou, Gao, Hongxia, Fan, Lipeng, Qiu, Songdong, Meng |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Wei sheng wu xue bao = Acta microbiologica Sinica. 53(8) |
| ISSN: |
0001-6209 |
| Popis: |
To investigate the effect of the Interferon-alpha-induced gp96 upregulation on the anti-HBV efficiency of Interferon-alpha (IFN-alpha).The effect of IFN-alpha on the transcription and expression of heat shock protein gp96 was determined by real-time PCR, luciferase reporter assay and Western blot. The effect of over-expression or knock-down of gp96 by transfection or RNA interference, and treatment with IFN-alpha on HBV expression and replication was examined by ELISA and RT-PCR.IFN-alpha treatment led to increased gp96 expression in a time- and dose-dependent manner. The overexpression of gp96 enhanced HBV expression and replication, whereas downregulation of gp96 resulted in decreased HBV replication. Finally, we verified that blocking IFN-alpha-induced upregulation of gp96 significantly enhanced IFN-alpha-mediated HBV inhibitory effects.IFN-alpha-induced upregulation of gp96 may negatively affect the anti-HBV function of IFN-alpha. These data provide valuable insight for enhancing the antiviral efficacy of IFN-alpha by simultaneous inhibition of gp96. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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