In vitro generation of whole osteochondral constructs using rabbit bone marrow stromal cells, employing a two-chambered co-culture well design
| Autor: | Kelei, Chen, Kian Siang, Ng, Sujata, Ravi, James C H, Goh, Siew Lok, Toh |
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| Rok vydání: | 2012 |
| Předmět: |
Compressive Strength
Tissue Scaffolds Silk Bone Marrow Cells Mesenchymal Stem Cells Bombyx Real-Time Polymerase Chain Reaction Coculture Techniques Extracellular Matrix Diffusion Calcification Physiologic Gene Expression Regulation Osteogenesis Animals Collagen Rabbits Peptides Tomography X-Ray Computed Cell Shape Chondrogenesis Glycosaminoglycans |
| Zdroj: | Journal of tissue engineering and regenerative medicine. 10(4) |
| ISSN: | 1932-7005 |
| Popis: | The regeneration of whole osteochondral constructs with a physiological structure has been a significant issue, both clinically and academically. In this study, we present a method using rabbit bone marrow stromal cells (BMSCs) cultured on a silk-RADA peptide scaffold in a specially designed two-chambered co-culture well for the generation of multilayered osteochondral constructs in vitro. This specially designed two-chambered well can simultaneously provide osteogenic and chondrogenic stimulation to cells located in different regions of the scaffold. We demonstrated that this co-culture approach could successfully provide specific chemical stimulation to BMSCs located on different layers within a single scaffold, resulting in the formation of multilayered osteochondral constructs containing cartilage-like and subchondral bone-like tissue, as well as the intermediate osteochondral interface. The cells in the intermediate region were found to be hypertrophic chondrocytes, embedded in a calcified extracellular matrix containing glycosaminoglycans and collagen types I, II and X. In conclusion, this study provides a single-step approach that highlights the feasibility of rabbit BMSCs as a single-cell source for multilayered osteochondral construct generation in vitro. |
| Databáze: | OpenAIRE |
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