| Autor: |
Yu-Jie, Bian, Wei-Wang, Li, Meng-Ke, Li, Bi-Chen, Wang, De-Yang, Shi, Jun, Shi, Wei-Ping, Yuan, Ya-Jing, Chu |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(2) |
| ISSN: |
1009-2137 |
| Popis: |
Objective: To establish an acquired aplastic anemia animal model for investigating the function of T lymphocyte and the pathogenesis and treatment of aplastic anemia(AA).To establish the acquired aplastic anemia mouse model through the X-ray irradiation in combination with lymphocytes injection. AA Group: the purified Pan T lymphocytes from the spleen of C57BL/6J mice were enriched and injected to the mice through tail vein(5×10Compared with 4, 5 Gy irradiated mice in AA groups, the survival time of 3 Gy irradiated AA groups was significantly prolonged. 3, 4 and 5 Gy X-ray irradiation combined with Pan T lymphocyte injection could successfully induced severe reduction of red blood cells, blood neutrophils, and platelets, severe reduction of bone marrow nucleated cells, severe bone marrow hematopoietic failure, and the significant expansion of T lymphocytes ratio in the bone marrow. CD43, 4 and 5 Gy X-ray irradiation combined with 5×10泛T淋巴细胞结合照射诱导的再生障碍性贫血小鼠模型的优化.建立适用于研究T淋巴细胞功能的获得性再生障碍性贫血(acquired aplastic anemia，AA)动物模型以及应用于再生障碍性贫血发病机制及治疗研究的动物模型.对X射线联合淋巴细胞注射诱导的获得性再生障碍性贫血小鼠模型进行优化。AA组：富集C57BL/6J小鼠的脾脏泛T细胞,按细胞数5×10相较于4、5 Gy照射的AA组，3 Gy照射的AA组生存期明显延长；经3、4、5 Gy X射线照射联合泛T淋巴细胞注射均可成功诱导受体鼠外周血红细胞、白细胞、血小板数严重减少，骨髓有核细胞严重减少，骨髓造血衰竭，骨髓中T淋巴细胞占比明显增加，CD43、4和5 Gy X射线的照射联合5×10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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