Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases

Autor: Prakash Kumar, Bhagat, Dipan, Roy, Ari, Sadanandom
Rok vydání: 2022
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2581
ISSN: 1940-6029
Popis: The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.
Databáze: OpenAIRE