| Autor: |
Dušan, Milanović, Patrick, Maier, Daniel H, Schanne, Frederik, Wenz, Carsten, Herskind |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 34(4) |
| ISSN: |
1791-7530 |
| Popis: |
13-cis-retinoic acid (RA) and thalidomide have shown a synergistic anti-proliferative effect on U343 glioblastoma (GBM) cells. In the present study, we test if their combined treatment might enhance the radiosensitivity of these cells.The radiosensitivity of U343 GBM cells was determined by the colony formation assay. Fibroblast growth factor-2 (FGF2) gene expression was determined by a quantitative polymerase chain reaction (qPCR).RA up-regulated FGF2 gene expression, which was abrogated by thalidomide. No radiosensitisation by RA was observed under standard culture conditions with 10% serum, but enhanced radiosensitivity was observed under 1% serum during irradiation. However, a synergistic effect with thalidomide was not observed.Growth factors in the culture medium may mask radiosensitization by RA while autocrine expression of FGF2 did not seem to be protective. Importantly, the anti-proliferative effect of RA in combination with thalidomide would not compromise the radiosensitivity of these GBM cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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