[The chromosome variability of the Indian muntjac in somatic cell hybrids]

Autor:	G G, Polianskaia, I I, Fridlianskaia
Rok vydání:	1991
Předmět:	Chromosome Aberrations Time Factors Deer Karyotyping Animals Genetic Variation Sarcoma Experimental Fibroblasts Hybrid Cells Cells Cultured Chromosomes Cell Line Rats
Zdroj:	Tsitologiia. 33(6)
ISSN:	0041-3771
Popis:	Hybrids were produced between the Indian muntjak fibroblasts and rat Jensen sarcoma cell line (JF1) auxotrophic for asparagine. They were selected without cloning under conditions providing survival of parental Indian muntjak and hybrid cells. This allowed to compare the Indian muntjak chromosome variability in the parental cells and hybrids under identical culture conditions. The frequency of muntjak chromosome aberrations proved to de higher in the hybrids (up to 47%) than in the parental cells (6.5%). Predominant are chromosomal breaks and dicentrics. The latter are mainly formed by fusion of chromosomes 1 and 2. The most fragile are 1 and X-chromosomes. Chromosomal breaks are evenly distributed along chromosome 1, and "hot" points are observed in X-chromosome. Possible mechanisms of the Indian muntjak chromosome rearrangements induced by somatic cell hybridization are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::bf0b5fdec936d75c786b3e7e7b6b4d0c https://pubmed.ncbi.nlm.nih.gov/1821494 Zobrazit plný text záznamu