[Detection of streptomycin resistance in Mycobacterium tuberculosis clinical isolates by denaturing high-performance liquid chromatography and DNA sequencing]
| Autor: | Rui-ru, Shi, Jian-yuan, Zhang, Xue-qin, Yuan, Zhao-gang, Sun, Chuan-you, Li |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
DNA Bacterial Ribosomal Proteins Adolescent DNA Mutational Analysis Reproducibility of Results Microbial Sensitivity Tests Mycobacterium tuberculosis Middle Aged Anti-Bacterial Agents Young Adult Bacterial Proteins Drug Resistance Bacterial Mutation Streptomycin Humans Tuberculosis Chromatography High Pressure Liquid Aged |
| Zdroj: | Zhonghua yi xue za zhi. 88(20) |
| ISSN: | 0376-2491 |
| Popis: | To determine the rpsL and rrs gene mutation in Mycobacterium tuberculosis (M. tuberculosis) and compare the consistency between the results of denaturing high-performance liquid chromatography (DHPLC) and those of DNA sequencing.The values of streptomycin minimum inhibitory concentration (MIC) against 215 M. tuberculosis clinical isolates, 115 being streptomycin-resistant and 100 being susceptible by a routine proportional method, were tested by DHPLC. DNA sequencing was conducted to detect the rpsL and rrs mutation.98 of the 115 streptomycin-resistant isolates (85.2%) harbored rpsL and/or rrs mutation, 76.5% of which being rpsL mutation (88/115). There was no significant correlation between the MIC values and mutation types. No mutation was found in all the susceptible isolates. There was a complete consistency between the DHPLC results and those of DNA sequencing.DHPLC can be regarded as a useful and powerful tool to detect the streptomycin resistance detection in M. tuberculosis. |
| Databáze: | OpenAIRE |
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