The levels of collagenase, tissue inhibitor of metalloproteinases-1 (TIMP-1), collagenase approximately TIMP-1 complexes and glycosaminoglycan (GAG) in sequential samples of synovial fluid aspirated from patients with osteoarthritis
| Autor: | T E, Cawston, V, Curry, S, Ramsey, I M, Clark, V A, Kyle, A, Adebajo, B, Silverman, T, Daymond, B L, Hazleman |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Knee Joint Anti-Inflammatory Agents Non-Steroidal Indomethacin Enzyme-Linked Immunosorbent Assay Tissue Inhibitor of Metalloproteinases Matrix Metalloproteinase Inhibitors Middle Aged Suction Naproxen Osteoarthritis Synovial Fluid Humans Female Collagenases Propionates Aged Glycoproteins Glycosaminoglycans |
| Zdroj: | Clinical and experimental rheumatology. 13(4) |
| ISSN: | 0392-856X |
| Popis: | Collagen turnover in connective tissues is thought to be controlled by the balance between the levels of interstitial collagenase and tissue inhibitor of metalloproteinases (TIMP-1). The aim of this study was to measure the level of total collagenase (MMP-1), TIMP-1, collagenase approximately TIMP-1 complex and glycosaminoglycan (GAG) in sequential samples of osteoarthritic knee synovial fluid from well documented patients to determine if these parameters changed with time and correlated with clinical indices.Twenty-one patients were recruited and randomly allocated to receive tiaprofenic acid, indomethacin or naproxen. Total collagenase, TIMP-1, collagenase approximately TIMP-1 complex and GAG were measured in 80 osteoarthritic synovial fluids taken over a period of six months.The majority of fluids contained a molar excess of TIMP-1 over collagenase, although in seven fluids collagenase was present in excess; six of these samples were from a single patient. GAG levels were relatively unchanged over the six months studied.The levels of collagenase and TIMP-1 varied between patients and over time in individual patients. No collagenase approximately TIMP-1 complex was found in any fluid. There was no significant difference in the median levels of collagenase, TIMP-1 or GAG in the different treatment groups. High levels of collagenase were found in one patient with a crystal related disease. These immunoassays give valuable information on the levels of collagenase and TIMP-1 in individual patients with time and may help to determine the mechanisms controlling the turnover of cartilage collagen in different arthritides. |
| Databáze: | OpenAIRE |
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