| Autor: |
Qu, Zheng, Wei, Liu, Guo-Wei, Zhang, Kai-Qiang, Li, Ling-Ling, Wang, Jing-Ping, Ge, Zhi-Qiang, Weng, Xue-Jun, Shang |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Zhonghua nan ke xue = National journal of andrology. 23(8) |
| ISSN: |
1009-3591 |
| Popis: |
To investigate the value of real-time RNA simultaneous amplification and testing (SAT) in the detection of Ureaplasma urealyticum (UU) in the semen of infertile males and its clinical significance.We collected semen samples from 542 infertility patients and 120 normal fertile men as controls in the Andrology Clinic of Nanjing General Hospital from March to September 2015. We detected UU infection in the samples using the culture method and SAT technology, respectively.All the UU positive cases (except 4 false positive cases) detected by the culture method were also shown to be positive in SAT. The UU detection rate of SAT was significantly higher than that of the culture method both in the infertility patients (54.1 vs 19.7%, P0.05) and in the normal controls (42.5 vs 12.5%, P0.05).SAT is a rapid and accurate method for detecting UU infection in semen samples, with a higher sensitivity and accuracy than the culture method, and it can also be used to evaluate the therapeutic effects. However, the culture method has its own advantages, such as low requirement of technical equipment, easy operation, and possibility of drug sensitivity test at the same time. Therefore, SAT and the culture method can be used alternatively according to the clinical need.目的： 探讨实时荧光RNA恒温扩增检测技术(SAT)在男性不育患者精液标本中检测解脲脲原体(UU)的效果和临床意义。 方法： 收集2015年3-9月精液标本共662例，其中不育患者542例，已生育生殖健康检查人群标本（生育对照组）120例，分别用培养法和SAT法同时检测以上标本。结果： 培养法阳性的标本（除4例假阳性标本除外）SAT法检测全为阳性，其中不育组培养法与SAT法UU检出率分别为19.7%与54.1%（P0.05），正常对照组为12.5%与42.5%(P0.05)。用SAT法检测不育组和生育对照组其阳性率分别为51.4%和42.5%（P0.05），而用培养法检测不育组和生育对照组的阳性率分别为19.7%和12.5%（P0.05）。SAT法和培养法的检出率分别为51.2%和18.4% (P0.05)，两种检测方法的总符合率为53.2%。结论： SAT技术可快速、准确检测UU的RNA，是未来发展方向，而培养法设备要求低，操作简单，可做药敏试验，应根据临床需要，灵活选用，提高UU的诊治水平。. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
