Oil biodegradation. Water droplets in oil are microhabitats for microbial life

Autor: Rainer U, Meckenstock, Frederick, von Netzer, Christine, Stumpp, Tillmann, Lueders, Anne M, Himmelberg, Norbert, Hertkorn, Philipp, Schmitt-Kopplin, Mourad, Harir, Riad, Hosein, Shirin, Haque, Dirk, Schulze-Makuch
Rok vydání: 2014
Předmět:
Zdroj: Science (New York, N.Y.). 345(6197)
ISSN: 1095-9203
Popis: Anaerobic microbial degradation of hydrocarbons, typically occurring at the oil-water transition zone, influences the quality of oil reservoirs. In Pitch Lake, Trinidad and Tobago--the world's largest asphalt lake--we found that microorganisms are metabolically active in minuscule water droplets (1 to 3 microliters) entrapped in oil. Pyrotag sequencing of individual droplet microbiomes revealed complex methanogenic microbial communities actively degrading the oil into a diverse range of metabolites, as shown by nuclear magnetic resonance and Fourier transform ion cyclotron resonance mass spectrometry. High salinity and water-stable isotopes of the droplets indicate a deep subsurface origin. The 13.5% water content and the large surface area of the droplets represent an underestimated potential for biodegradation of oil away from the oil-water transition zone.
Databáze: OpenAIRE