| Autor: |
O A, Pavlish, N B, Seniuta, L N, Shcherbak, N V, Klitsunova, M S, Shtutman, O E, Pomekhin, V E, Grutsevich |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Voprosy virusologii. 45(2) |
| ISSN: |
0507-4088 |
| Popis: |
Three HTLV-I-infected partially interleukin-2-dependent lymphoid cell lines were derived from a patient with T-cellular leukemia (ATL) from Georgia and a carrier of HTLV-I from Sakhalin. The strains cultured in the presence of 3-5% interleukin-2 were designated as NBK-1, NBK-2, and YE-1, respectively. Immunoblotting analysis showed typical HTLV-I proteins in them except NBK-2 which expressed nontypical proteins p40K and p28-29K. Unexpectedly, leukemic cell fraction ATL/NBK from a patient contained gag proteins p19 (CA), p24 (MA), Pr53, and unidentified protein p29. Southern blot analysis of primary leukemic cells NBK showed one full-length non-defective provirus with restriction sites Sacl in both LTRs. Limited restriction map of the provirus virtually did not differ from previously described HTLV-I prototypes. Although the mechanism of abnormal protein expression remains to be determined, this event can be explained by defective provirus formation in NBK-2 cell line during coculturing of leukemic cells with human umbilical cord blood lymphocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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